RAK4630 not connecting to LoraWan

Thank you Bernd.
I tried to use this new example but I updated the 4630 firmware with the WistoolBox and updated it to RUI.
After this I can not load any other firmware anymore!

I get this error every time I try to load any new sketch even the one who was working before.

Failed to upgrade target. Error is: No data received on serial port. Not able to proceed.
Traceback (most recent call last):
File “main.py”, line 296, in serial
File “dfu\dfu.py”, line 235, in dfu_send_images
File “dfu\dfu.py”, line 199, in _dfu_send_image
File “dfu\dfu_transport_serial.py”, line 179, in send_start_dfu
File “dfu\dfu_transport_serial.py”, line 243, in send_packet
File “dfu\dfu_transport_serial.py”, line 282, in get_ack_nr
nordicsemi.exceptions.NordicSemiException: No data received on serial port. Not able to proceed.

Possible causes:

  • Selected Bootloader version does not match the one on Bluefruit device.
    Please upgrade the Bootloader or select correct version in Tools->Bootloader.
  • Baud rate must be 115200, Flow control must be off.
  • Target is not in DFU mode. Ground DFU pin and RESET and release both to enter DFU mode.

I also try to update the bootloader with instructions I found here Updated bootloader for RAK4631 - #5 by richstimson

but it also gave me errors:
C:\Users\Santiago H\Downloads>adafruit-nrfutil.exe --verbose dfu serial --package RAK4630_bootloader-0.4.1.zip --port COM27 -b 115200 --singlebank --touch 1200
Upgrading target on COM27 with DFU package C:\Users\Santiago H\Downloads\RAK4630_bootloader-0.4.1.zip. Flow control is disabled, Single bank, Touch 1200

Failed to upgrade target. Error is: Serial port could not be opened on COM27. Reason: could not open port ‘COM27’: PermissionError(13, ‘Acceso denegado.’, None, 5)
Traceback (most recent call last):
File “dfu\dfu_transport_serial.py”, line 99, in open
File “serial\serialwin32.py”, line 33, in init
File “serial\serialutil.py”, line 244, in init
File “serial\serialwin32.py”, line 64, in open
serial.serialutil.SerialException: could not open port ‘COM27’: PermissionError(13, ‘Acceso denegado.’, None, 5)

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
File “main.py”, line 296, in serial
File “dfu\dfu.py”, line 226, in dfu_send_images
File “dfu\dfu.py”, line 157, in _dfu_send_image
File “dfu\dfu_transport_serial.py”, line 101, in open
nordicsemi.exceptions.NordicSemiException: Serial port could not be opened on COM27. Reason: could not open port ‘COM27’: PermissionError(13, ‘Acceso denegado.’, None, 5)

Possible causes:

  • Selected Bootloader version does not match the one on Bluefruit device.
    Please upgrade the Bootloader or select correct version in Tools->Bootloader.
  • Baud rate must be 115200, Flow control must be off.
  • Target is not in DFU mode. Ground DFU pin and RESET and release both to enter DFU mode.

I also try with BLE and nothings happens.

I can not see any device callaed RAK4160_OTA when I try to connect it by BLE.